Services

List of services

The Facility offers the following services to all researchers for a fee:

  1. DNA Sequencing (Sanger, Capillary)
  2. Genotyping (Fragment Analysis and High Resolution Melt Analysis)
  3. Real-time PCR
  4. Promoter Characterization (MSACE and DFACE)
  5. SHAPE analysis
  6. Microplate Reader
  7. Biomolecular Interaction Analysis
  8. Robotics (Liquid handling applications)
  9. Education (tours, lectures and demonstrations; free)

General Information

Initial Consultaton

For all services, other than Sanger Capillary DNA Sequencing, we require a personal consultation or at least a telephone conversation in order to discuss your project prior to beginning. There are three primary reasons for doing this. First, our understanding of your objectives, resources, and schedule is critical for planning the experiments. Second, it is important for you to understand the options, advantages and limitations of the facility's instruments and services. Third, there are numerous details, e.g. DNA concentration, buffer composition, etc., that need to be decided upon prior to proceeding with research at the Facility. There are no charges for this initial consultation.

Troubleshooting

For all services, PMGF happily offers it's services in troubleshooting and interpreting data generated at the Facility. Often times, this can be done without scheduling an appointment, although larger and more involved data sets will require a scheduled meeting. Please feel free to come to the lab, call, or email to address any issues that you may encounter.

Novel Applications

The Facility is always interested in performing novel applications on the instrumentation here or adapting established experimental procedures to fluorescent and/or high throughput technology. For example, we have adapted DNA Footprint Analysis so it can be performed on an automated capillary DNA Analyzer. Please contact us if you would like to explore a novel use of instrumentation in the Facility.

Publication Acknowledgement Guidelines

If you are using data generated by the PMGF for a publication, it is requested that you consider carefully whether to acknowledge or include as authors the PMGF staff involved in generating the data. Please consider authorship when appropriate, as it is highly valuable in professional development of the Resource Facility staff and recognizes the significant support of the Facility by the College of Arts and Sciences.

Authorship - The Association of Biomolecular Resource Facilities (ABRF) has published guidelines to help in this determination. The “Recommended Guidelines for Authorship on Manuscripts” can be found here:  http://www.abrf.org/index.cfm/page/resources/Authorship.htm.
Acknowledgement – Any data generated by the PMGF that is used for publication, either in the manuscript or as supplementary data made available by the publisher of the manuscript should contain the following in the acknowledgments (or equivalent) section of the manuscript:

 “We thank the Plant-Microbe Genomics Facility at The Ohio State University for their contribution to this publication.”

Data Retention Policy

All data generated on and after January 1, 2009 is retained at the Facility and can be retrieved upon request by the client with the following exception.  Massively Parallel Pyrosequencing data will be retained for a minimum of two years, although it may be longer depending upon capacity. For the years 2000 - 2008 all of the data files were backed up, but some files are gone due to errors and optical media failure although a large majority are available upon request.

 

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