Biomolecular Interaction Analysis (Discontinued)

diagram of surface plasmon resonanceThe Facility has a GE Healthcare Biacore T100 that can measure the interaction between proteins and proteins, DNA and proteins, metabolites and proteins, as well as other biomolecules that can be anchored to a gold covered chip (comprehensive list [pdf]). The measurements are done in real-time with no labels and utilize surface plasmon resonance (SPR) technology. The T100 can measure concentration, binding specificity, and affinity constants.

Due to the nature of the studies performed with the T100, the client uses the instrument in order to allow for instant analysis of the results and therefore immediate decisions about the next step or experiment to be done.  In order to use the instrument, all clients are required to attend a training session provided by the Facility or the Biacore Basics course offered by GE Healthcare. The training provided by the staff is done at the Facility, takes one to three days, and can accommodate up to 2 clients at a time. The client will need to provide all materials and training will utilize the client's samples. An experienced staff member will provide guidance during the initial experiment and the training has a set fee. The training will include: practical use of the instrument, experimental design, and analysis. Please contact the Facility to arrange a training session and begin using the Biacore T100.

The Facility has the following software to aid in the analysis of the data generated by the Biacore T100: Biacore T100 Evaluation Software. 

Example of results

Order and Instrument Reservation

How to place an order

  1. Log into dnaLIMS (or create an account if you have not done so previously)
  2. Contact us at pmgf@osu.edu or 614-247-6204 to schedule an appointment
  3. Make the order
    1. Click on "Other Services" tab.
    2. Click on the "Biomolecular Interaction Analysis" form and follow the instructions.
  4. Make an eRequest (OSU customers only).

How to obtain results

  1. Download the files from the Biacore computer to a flash drive
  2. Email the results to yourself using a web-based email service from the Biacore computer

[pdf] - Some links on this page are to .pdf files. These are designated by [pdf] following the link. If you require these materials in alternate format contact our web manager.

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